This month the zeta potential analysis of HA/Chi multilayer coatings on glass slides with 14 mm x 10 mm size was performed. The pH dependence of zeta potential and the isoelectric point derived there from do not enable a differentiation of the PEM with different terminal layers. Neither does an effect of cross-linking become visible by the zeta potential results. We conclude that an intermixing between adjacent HA and Chi layers at a higher number of polysaccharide bilayers makes the outermost surface indistinguishable in terms of surface functional groups. Such behavior is already reported in the literature for weak polyelectrolytes such as hyaluronic acid or chitosan.